Coding

Part:BBa_I745005:Design

Designed by: MGC collection   Group: iGEM07_Princeton   (2007-10-26)


Bak gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 148
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 136


Design Notes

We PCR'd the gene out of the plasmid and added SfiI sites to either end.


Source

ATCC, mouse.

References